This method was first described by Trinder in 1969 thus named after him, (Lott et al, 1975). glucose oxidase and hexokinase etc.) After forty minutes, the solutions changed colour from colourless to light pink according to the concentration. Collect fresh urine specimen in a clean dry container with labeling. Glucose Test Strips 101: Uses, Accuracy, Cost & More - Healthline The Yellow Springs glucose analyzer, which continues to be used in clinical laboratories, was the first to exploit this methodology more than 30 years ago [17]. What are the advantages of using the enzymatic method for the determination of blood glucose. A speck of culture is rubbed on it with a platinum loop. Different methods based on different properties of glucose are described for blood glucose estimation. Glucose vs. plasma glucose: Glucose levels in plasma (one of the components of blood) are higher than glucose measurements in whole blood; the difference is about 11% when the hematocrit is normal. Add 0.2 ml of 1% -naphthol, then add 0.3 ml of 1% p-aminodimethylaniline oxalate (Gaby and Hadley reagents). Observe for color change within 10 seconds. nature of the reaction must not be mycelial . What are the advantages and disadvantages of a glucose oxidase-based glucose sensor . Add approximately 10 \(\mu\)L of the hemolysate to 1 mL of a sample whose glucose was previously measured. Using appropriate cuvettes, measure the absorbance of each reaction mixture at 525 nm against a water blank. Glucose meter - Wikipedia It doesn't target other sugars except glucose. 2008-2021 ResearchGate GmbH. This method gives much lower blood glucose values with blood samples of neonatal jaundice (plasma bilirubin level > 10 mg/dL) of age 10 +/- 5 daysthan with samples of neonates of the same age group without jaundice or older children suffering from other . Advantages of the Folin-Wu Method for Blood Glucose Estimation Blood glucose level estimation can play a significant role in diabetic management; it helps doctors decide whether to give insulin and provides information on glycemic control.
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advantages and disadvantages of glucose oxidase method